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BTN3A1 Antibody
SKU: orb1174074
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, WB |
|---|---|
| Dilution Range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM94 |
| Target | BTN3A1 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BTN3A1, BTF5, CD277, BTN3.1, BT3.1
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human BTN3A1 protein, mFc-His tagged protein orb689408 can bind Rabbit anti-BTN3A1 monoclonal antibody (clone: DM94) in a linear range of 0.64-80 ng/ml.
Flow cytometry analysis with Anti-BTN3A1 on Expi293 cells transfected with human BTN3A1 (Red histogram) or Expi293 transfected with irrelevant protein (Blue histogram).
Flow cytometry analysis of antigen binding of rabbit anti-human BTN3A1 mAb (orb1174074). (A) orb1174074 does not bind to MCF-7 cells that do not express BTN3A1. (B) A clear peak shift of orb1174074 was seen compared to the control when incubated with BTN3A1-expressing 8226 cells, indicating strong binding of orb1174074 to BTN3A1. Antibodies were incubated at 2 μg/mL.
Anti-BTN3A1 antibody (SKU# orb1174074) at 1/1000 dilution.Lane: RPMI8226, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 33 kDa.Observed band size: 35, 54, 68 kDa
Quick Database Links
Gene Symbol
BTN3A1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
BTN3A1 Antibody (orb1174074)
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