Galactose Kinase Rabbit Polyclonal Antibody

SKU: orb213966

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Description

The Galactose Kinase Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Galactose Kinase. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Pharmacology & Drug Discovery

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC, WB
Dilution Range	WB: 1-500-1-1000, IF/ICC: 1-100-1-500
Reactivity	Canine, Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Galactose Kinase. The exact sequence is proprietary.
Target	GALK1
Molecular Weight	42 kDa
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GALK; Galactokinase; Galactose kinase

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Galactose Kinase Rabbit Polyclonal Antibody

Immunofluorescent analysis of Galactose Kinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of mouse lung (1), mouse liver (2), mouse kidney (3), rat liver (4) using Galactose Kinase Rabbit Polyclonal Antibody orb213966 with dilution at 1:1000.

Immunohistochemical analysis of paraffin-embedded human lung cancer using Galactose Kinase Rabbit Polyclonal Antibody orb213966 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat kidney using Galactose Kinase Rabbit Polyclonal Antibody orb213966 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat spleen using Galactose Kinase Rabbit Polyclonal Antibody orb213966 with dilution at 1:100.

Quick Database Links

Gene Symbol

GALK1

UniProt

Q9R0N0, P51570

NCBI Gene ID

2584, 14635

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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