Anti-Ly6G/Ly6C [RB6-8C5]

SKU: orb758800

Description

Rat monoclonal antibody to Ly6G

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	FC, IHC, WB
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	RB6-8C5
Immunogen	This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
Target	Ly6G/Ly6C
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Gr-1; Gr1; Ly-6G; ly-6G.1; ly6C

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Flow-cytometry using the Anti-Ly6G/Ly6C antibody RB6-8C5. Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RB6-8C5 (orb758799, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes using anti-Ly6G/Ly6C antibody RB6-8C5. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of RB6-8C5 (orb758799) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758799, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Ly6G/Ly6C

UniProt

P35461

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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