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p38 (pT180) Antibody
SKU: orb2651002
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IP, WB |
|---|---|
| Dilution range | WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), CHIP: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), E: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) |
| Reactivity | Human, Mouse, Rat, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Clone No. | MAPK14 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a
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Human, Mouse, Rat, Zebrafish
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Polyclonal
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50 μl 
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Western blot analysis of p38 (pT180) expression in zebrafish (A) whole cell lysates.
Western blot analysis of p38 (pT180) expression in mouse liver (A), rat liver (B) whole cell lysates.
Direct ELISA antibody dose-response curve using Anti-p38 (pT180) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Immunofluorescent analysis of p38 (pT180) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
p38 (pT180) Antibody (orb2651002)
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