Anti-Rat antibody kappa light chains [OX-12]

SKU: orb2645680

This product can be custom made

Description

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, FC, IF, IHC, IP, RIA, WB
Reactivity	Rat

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	OX-12
Immunogen	OX-12 was generated by immunizing BALB/c mice with purified rat F(ab')2.
Target	Rat antibody kappa light chains
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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kappa Lc; OX12; MRC OC-12; MRC OX12

Quality Guarantee

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Anti-Rat antibody kappa light chains [OX-12]

Flow cytometry using the Rat antibody kappa light chains antibody OX-12. Paraformaldehyde fixed rat splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of OX-12 (orb2645680, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed rat splenocytes with anti-Rat kappa light chains antibody OX-12. Immunofluorescence analysis of paraformaldehyde fixed rat splenocytes on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OX-12 (orb2645680) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645680, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using Anti-Rat antibody kappa light chains antibody OX-12. Rat spleen tissue lysate (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OX-12 (orb2645680) 0.001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.