Arginase Antibody

SKU: orb749619

Description

This protein is involved in the pathway that synthesizes L-ornithine and urea from L-arginine.

Research Area

Epigenetics, Immunology

Images & Validation

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Item 1 of 4

Tested Applications	IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the Arginase antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG3, kappa
Clone No.	ARG1/1126
Immunogen	A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC: Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Arginase antibody (ARG1/1126). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT prior to testing.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Arginase antibody (clone ARG1/1126). These results demonstrate the foremost specificity of the ARG1/1126 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Arginase antibody (clone ARG1/1126) as confirmation of integrity and purity.

Western blot analysis of human liver lysate using Arginase 1 antibody (ARG1/1126). Predicted molecular weight ~35 kDa.

Quick Database Links

UniProt

P05089

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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