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c-Kit rabbit pAb
SKU: orb768898
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Reactivity | Human, Mouse |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human KIT. AA range:906-955 |
| Molecular Weight | 117 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-KIT antibody, anti-SCFR antibody, anti-Mast/stem cell growth factor receptor Kit antibody, anti-SCFR antibody, anti-Piebald trait protein antibody, anti-PBT antibody, anti-Proto-oncogene c-Kit antibody, anti-Tyrosine-protein kinase Kit antibody, anti-p145 c-kit antibody, anti-v-kit Hardy-Zuckerman 4 feline sarcoma viral onco antibody
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Western Blot analysis of 453 cells using c-Kit Polyclonal Antibody diluted at 1:500.
Western blot analysis of lysates from COLO205 cells, using KIT Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from K562 cells using KIT antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
c-Kit rabbit pAb (orb768898)
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