c-Kit (phospho Tyr721) rabbit pAb

SKU: orb764164

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Description

Rabbit polyclonal antibody to c-Kit (phospho-Tyr721)

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution range	Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human c-Kit around the phosphorylation site of Tyr721. AA range:688-737
Molecular Weight	110
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-KIT antibody, anti-SCFR antibody, anti-Mast/stem cell growth factor receptor Kit antibody, anti-SCFR antibody, anti-Piebald trait protein antibody, anti-PBT antibody, anti-Proto-oncogene c-Kit antibody, anti-Tyrosine-protein kinase Kit antibody, anti-p145 c-kit antibody, anti-v-kit Hardy-Zuckerman 4 feline sarcoma viral onco antibody

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Western Blot analysis of various cells using Phospho-c-Kit (Y721) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using c-Kit (Phospho-Tyr721) Antibody.

Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using c-Kit (Phospho-Tyr721) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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