Calprotectin, Human, mAb 27E10, Biotin Antibody

SKU: orb1108920

Description

Mouse monoclonal antibody against Calprotectin conjugated to Biotin

Images & Validation

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Tested Applications	FC, IA, IF, IHC-Fr, IP, WB
Dilution range	Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Reactivity	Human
Application Notes	W: non-reduced;~22 kDa; doesn€t recognize the single proteins S100A8 and S100A9 HPLC: reduced (~17 kDa) and non-reduced(various sizes due to association with other elements (Ref1) F: acetone fixation; 0.1 % hydrogen peroxide treatment to reduce enogenous peroxidise activity; positive control: inflammatory tissue; negative control: normal human tissue (skin, lung, colon) (Ref1) FC: Extracelullar expression on monocytes, as negative control HL-60, platelets, lymphocytes can be used. Read more...

Key Properties

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Clonality	Monoclonal
Isotype	Mouse IgG1
Immunogen	Human blood monocytes
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	0.5 ml (100 µg/ml) 0.2 um filtered biotinylated protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Buffer/Preservatives	0.5 ml (100 μg/ml) 0.2 μm filtered biotinylated protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Disclaimer	For research use only

Alternative Names

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S100A8/A9, MRP-8/MRP-14, calprotectin, calgranulin-A/calgranulin-B, L1-protein

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence

Immunoprecipitation