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Description
Images & Validation
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| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:25 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This CARD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CARD6. |
| Target | CARD6 |
| Molecular Weight | 116468 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Caspase recruitment domain-containing protein 6; CARD6
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Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from A549, HepG2, A431, HL-60, THP-1 cell line (from left to right), using CARD6 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_115976.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
CARD6 Antibody (Center) (orb1788174)
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