CCL20/MIP-3-Alpha Antibody

SKU: orb1538966

Description

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, WB
Dilution range	ELISA (2 - 4 µg/ml), IHC, IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml)
Reactivity	Human
Application Notes	Resuspension: Centrifuge the vial before opening. Reconstitute in sterile water to 0.1 mg/ml.. Further information: Immunohistochemistry: LS-B7409 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3alpha when used with biotinylated antigen affinity purified anti-Human MIP-3alpha (LS-C104612) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 0.25-0.50 ng/lane, under non-reducing conditions. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1,k
Immunogen	E.coli derived recombinant Human MIP-3alpha.
Target	CCL20 / MIP-3-Alpha
Purification	Protein A purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Lyophilized from PBS. Lyophilized from PBS
Disclaimer	For research use only

Alternative Names

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CCL20, Beta chemokine exodus-1, Beta-chemokine exodus-1, CKb4, Exodus-1, MIP-3a, MIP-3-alpha, LARC, ST38, SCYA20, C-C motif chemokine 20, CC chemokine LARC, Mip-3alpha, MIP3A, Small-inducible cytokine A20

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol

CCL20 / MIP-3-Alpha

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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