CD135/FLT3 Antibody

SKU: orb1534432

Description

CD135/FLT3 Antibody

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P
Dilution range	ELISA, IHC, IHC-P (8 µg/ml)
Reactivity	Human
Application Notes	Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 8 ug/ml. Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic 20 amino acid peptide from internal region of human FLT3 / CD135. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Rabbit (95%); Hamster (85%); Bovine, Dog, Panda, Horse (80%).
Target	CD135 / FLT3
Purification	Immunoaffinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, 0.1% Sodium Azide. PBS, 0.1% Sodium Azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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FLT3, CD135, CD135 antigen, Fetal liver kinase 2, Fetal liver kinase-2, FL cytokine receptor, FLK2, FLT-3, Fms-like tyrosine kinase 3, Stem cell tyrosine kinase 1, STK-1, FLK-2, Fms-related tyrosine kinase 3, STK1

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Quick Database Links

Gene Symbol

CD135 / FLT3

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Datasheet

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Protocol Information

IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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