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CD33 Rabbit Polyclonal Antibody

SKU: orb2563093

Description

CD33 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsFC, WB
Dilution RangeWB=1:500-2000, Flow-Cyt=1ug/Test
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant human CD33 protein (18-259/364aa)
TargetCD33
Molecular Weight67 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD33rSiglec; SIGLEC-3; SIGLEC3; p67; gp67; CD33_HUMAN; CD33; Sialic acid-binding Ig-like lectin 3 (Siglec-3); CD33_MOUSE;

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CD33 Rabbit Polyclonal Antibody

20 ng rhCD33-His protein per lane probed with CD33 polyclonal antibody respectively, unconjugated (orb2563093) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

CD33 Rabbit Polyclonal Antibody

The THP-1 (H) cells incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD33 antibody (orb2563093): 1 µg/10^6 cells, Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 µg/Test. Isotype Control (orange): Rabbit IgG (orb570250). Blank control (black): PBS. Acquisition of 20000 events was performed.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol

CD33 Rabbit Polyclonal Antibody (orb2563093)

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Available Sizes

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50 μl
¥ 3,250.00
100 μl
¥ 4,810.00
200 μl
¥ 6,110.00
DispatchUsually dispatched within 5-10 working days
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