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Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1). |
| Target | CD4 |
| Molecular Weight | Observed: 51kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD4 Antibody [9H5A8] : CD4mut
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Western blot analysis of extracts of U-937 cells, using CD4 antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded rat thymus using CD4 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using GRP78 / BiP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
CD4
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
CD4 Antibody (orb1261848)
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