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CD63 Antibody (C-term)
SKU: orb1930560
Description
Images & Validation
−Item 1 of 7
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:1000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000, WB: 1:2000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB26813 |
| Target | This CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-190 amino acids from the C-terminal region of human CD63. |
| Molecular Weight | 25637 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30
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Anti-CD63 Antibody (C-term) at 1:2000 dilution + human plasma lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CD63 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CD63 Antibody (C-term) at 1:1000 dilution. Lane 1: A2058 whole cell lysates. Lane 2: MDA-MB-453 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: MDA-MB-453 whole cell lysate. Lane 2: A2058 whole cell lysate. Lane 3: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: MDA-MB-453 whole cell lysate. Lane 2: A2058 whole cell lysate. Lane 3: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: U-251 MG whole cell lysate. Lane 3: THP-1 whole cell lysate. Lane 4: A2058 whole cell lysate. Lane 5: A375 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilution. Lane 1: A2058 whole cell lysates. Lane 2: A375 whole cell lysates. Lane 3: HL-60 whole cell lysates. Lane 4: THP-1 whole cell lysates. Lane 5: U-87 MG whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
This CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-190 amino acids from the C-terminal region of human CD63.
UniProt
RefSeq (Protein):NP_001244330.1, NP_001771.1, NP_001244318.1, NP_001244320.1, NP_001244329.1, NP_001244321.1, NP_001254627.1, NP_001244319.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein productDocuments Download
Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
CD63 Antibody (C-term) (orb1930560)
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