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CD74 Antibody
SKU: orb2641954
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FACS, IF, IHC |
|---|---|
| Dilution range | Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry: 1-2ug/ml,Prediluted IHC only format: incubate for 30 min at RT (1) |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | CDLA74-1 |
| Immunogen | Recombinant full-length human protein was used as the immunogen for the CD74 antibody. The epitope is known to be extracellular. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Flow cytometry testing of human Raji cells with CD74 antibody (clone CDLA74-1); Red = isotype control, Blue = CD74 antibody.
Immunofluorescent staining of human Raji cells with CD74 antibody (green, clone CDLA74-1) and Reddot nuclear stain (red).
IHC testing of FFPE human tonsil with CD74 antibody (clone CDLA74-1). HIER: FFPE testing requires sections to be boiled in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
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Protocol Information
IHC
Immunohistochemistry
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
CD74 Antibody (orb2641954)
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