Chicken Rabbit IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse IgG Antibody

SKU: orb700356

Description

Gallus polyclonal antibody against Gallus anti-Rabbit IgG (H&L) conjugated to AP

Images & Validation

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Tested Applications	ELISA, ICC, IHC, WB
Dilution range	1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Reactivity	Rabbit
Application Notes	This antibody reacts with the heavy chains on rabbit IgG and the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins or to human or mouse serum or IgG based on immunoelectrophoresis.

Key Properties

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Host	Gallus
Clonality	Polyclonal
Immunogen	Purified Rabbit IgG
Purification	Immunogen affinity purified chicken IgY.
Conjugation	ALP

Storage & Handling

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Storage	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/Appearance	Liquid
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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