Chicken Rabbit IgG (H&L), Affinity purified, Unconjugated, min, cross-reactivity to human, mouse IgG Antibody

SKU: orb700355

Description

Gallus polyclonal antibody to Gallus anti-Rabbit IgG (H&L)

Images & Validation

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Tested Applications	ELISA
Dilution range	The optimal working dilution should be determined by the investigator
Reactivity	Rabbit
Application Notes	Antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum and human or mouse serum or IgG based on immunoelectrophoresis. Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml

Key Properties

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Host	Gallus
Clonality	Polyclonal
Immunogen	Purified Rabbit IgG
Purity	Affinity purified chicken IgY
Purification	Immunogen affinity purified chicken IgY.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store non-diluted antibody at 2-8°C, For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity, Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming,!!!Store non-diluted antibody at 2-8°C, For storage at -20°C dilute with an equal volume of glycerol to prevent loss of enzymatic activity, Prepare working dilution prior to use and then discard,Shelf life is 1 year from date of receipt,
Form/Appearance	Liquid
Disclaimer	For research use only

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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