CREB-1 Mouse Monoclonal Antibody

SKU: orb499769

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Description

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	6B4
Immunogen	Recombinant human CREB-1
Target	CREB1
Molecular Weight	37 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB 1; CREB1; MGC9284; Transactivator protein; CREB.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of orb499769) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of orb499769 6B4) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CREB-1) Monoclonal Antibody, Unconjugated (ascites of orb499769) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Huvec (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, Primary: Anti-CREB-1 (orb499769) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 43 kD.

Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ug, Primary: Anti-CREB-1 (orb499769) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 45 kD.

Quick Database Links

Gene Symbol

CREB1

UniProt

P16220

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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