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Cyclin B1 Recombinant Rabbit Monoclonal Antibody
SKU: orb559089
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 2E6 |
| Immunogen | Recombinant human Cyclin B1 protein（1-150aa） |
| Target | CCNB1 |
| Molecular Weight | 48 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclin B1) Monoclonal Antibody, Unconjugated (orb559089) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Hela cell lysate, Lane 2: Daudi cell lysate, Lane 3: K562 cell lysate, Primary: Anti-Cyclin B1 (orb559089) at 1/500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1/5000 dilution, Predicted band size: 48 kD, Observed band size: 55 kD.
Quick Database Links
Gene Symbol
CCNB1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
Cyclin B1 Recombinant Rabbit Monoclonal Antibody (orb559089)
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