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Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:10~50 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This EIF3H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human EIF3H. Antigen Region: 70-99 aa. |
| Target | EIF3H {ECO:0000255|HAMAP-Rule:MF_03007} |
| Molecular Weight | 39930 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Eukaryotic translation initiation factor 3 subunit H {ECO:0000255|HAMAP-Rule:MF_03007}, eIF3h {ECO:0000255|HAMAP-Rule:MF_03007}, Eukaryotic translation initiation factor 3 subunit 3 {ECO:0000255|HAMAP-Rule:MF_03007}, eIF-3-gamma, eIF3 p40 subunit {ECO:0000255|HAMAP-Rule:MF_03007}, EIF3H {ECO:0000255|HAMAP-Rule:MF_03007}
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EIF3H Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of EIF3H Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-EIF3H Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: RD whole cell lysates. Lane 3: PC-12 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003747.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
EIF3H Antibody (N-term) (orb1788098)
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