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EREG Antibody (C-term)
SKU: orb1930813
Description
Images & Validation
−Item 1 of 7
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | IHC-P - 1:100-500, FC - 1:25, WB - 1:2000 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 19044 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
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Human
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
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Anti-EREG Antibody (C-term) at 1:2000 dilution + A549 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-EREG Antibody (C-term) at 1:2000 dilution + U-87 MG whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysates from A549, HepG2, U-87 MG cell line (from left to right), using EREG Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
Staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
EREG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EREG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of EREG Antibody (C-term) in HepG2 cell line lysates (35 ug/lane). EREG (arrow) was detected using the purified Pab.
All lanes: Anti-EREG Antibody (C-term) at 1:1000 dilution. Lane 1: A549 whole cell lysates. Lane 2: human placenta lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
EREG Antibody (C-term) (orb1930813)
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