F(ab')2 Rat IgG F(ab')2 Antibody Peroxidase Conjugated

SKU: orb346799

Description

F(ab')2 Rat IgG F(ab')2 antibody (Peroxidase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Reactivity	Rat
Application Notes	F(ab')2 Anti-Rat IgG F(ab')2 Peroxidase Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Rat IgG F(ab')2 fragment
Purity	This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	10 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit F(ab')2 anti-Rat IgG F(ab')2 peroxidase Conjugated Antibody, rabbit F(ab')2 anti-Rat IgG F(ab')2 HRP Conjugated Antibody

Rat IgG F(ab')2 Peroxidase Antibody [orb346349]

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Source:	Rat

Sizes Available: 1 mg

F(ab')2 Rat IgG (H&L) Antibody Peroxidase Conjugated [orb346798]
Applications:
ELISA, IHC, WB
Reactivity:
Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
HRP
Sizes Available: 20 mg
F(ab')2 Rat IgG (H&L) Antibody Peroxidase Conjugated [orb346793]
Applications:
ELISA, IHC, WB
Reactivity:
Rat
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
HRP
Sizes Available: 20 mg

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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