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Gab1 Antibody (Ascites)
SKU: orb652191
Description
Research Area
Cancer Biology, Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | WB |
|---|---|
| Dilution range | WB: 1:1000 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Target | GAB1 |
| Molecular Weight | 76616 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Anti-GRB2-associated-binding protein 1 antibody, anti-GRB2-associated binder 1 antibody, anti-Growth factor receptor bound protein 2-associated protein 1 antibody, anti-GAB1 antibody
Quality Guarantee
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Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent. After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells.
Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42°C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 Antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with anti-Gab1 Antibody.
Quick Database Links
Gene Symbol
GAB1
UniProt
UniProt Details
− No UniProt data available
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Gab1 Antibody (Ascites) (orb652191)
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