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GAPDH Antibody
SKU: orb1939451
Description
Research Area
Cancer Biology, Metabolism Research, Microbiology, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500, IF - 1:25 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Molecular Weight | 36053 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
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All lanes: Anti-GAPDH Antibody at 1:1000 dilution + C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 36KDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-GAPDH Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: C6 whole cell lysates. Lane 3: Hela whole cell lysates. Lane 4: HUVEC whole cell lysates.Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-GAPDH Antibody at 1:8000 dilution. Lane 1: Hela whole cell lysates. Lane 2: A549 whole cell lysates. Lane 3: COS-7 whole cell lysates. Lane 4: mouse brain lysates. Lane 5: C6 whole cell lysates. Lane 6: NIH/3T3 whole cell lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody. diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of anti-GAPDH Monoclonal Antibody in CEM cell line lysates (35 μg/lane). GAPDH (arrow) was detected using the purified Mab.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
GAPDH Antibody (orb1939451)
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