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KPNA2/IPOA1 Antibody
SKU: orb18623
Description
Research Area
Epigenetics, Infectious Diseases
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml |
| Reactivity | Human, Rat |
| Predicted Reactivity | Canine, Mouse |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | KPNA2 / IPOA1 |
| Protein Sequence | QVQDGAPGTFNF |
| Molecular Weight | 57.9 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti KPNA2 antibody, anti IPOA1 QIP2 antibody, anti RCH1 antibody, anti SRP1alpha antibody, anti karyopherin alpha 2 (RAG cohort 1, importin alpha 1) antibody, anti RAG cohort 1 antibody, anti importin alpha 1 antibody, anti importin alpha 2 antibody, anti importin-alpha-P1 antibody, anti karyopherin alpha 2 antibody, anti pendulin antibody
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5 μg/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
0.1 μg/ml staining of Jurkat (A) and CaCo-2 (B) and (0.03 μg/ml) A549 (C), MCF7 (D) and KNRK (E) cell lysate. (35 μg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and ER/Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
KPNA2/IPOA1 Antibody (orb18623)
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