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NOVA1 Antibody
SKU: orb19796
Description
Research Area
Epigenetics, Neuroscience
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 50-55kDa band observed in Human Breast Cancer lysates, and in preliminary testing of Human Kidney lysate (calculated MW of 51.7kDa according to NP_002506.2). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5µg/ml. Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | NOVA1 |
| Molecular Weight | 51.7, 19.5 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti NOVA1 antibody, anti neuro-oncological ventral antigen 1 antibody, anti Nova-1 antibody, anti paraneoplastic Ri antigen antibody, anti ventral neuron-specific protein 1 antibody
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Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Primary incubation 1 hour at room temperature. Image A: Human Breast Cancer lysate at primary Ab concentration 0.03 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
5 µg/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH6, AP-staining.
5 µg/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH6, AP-staining.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
NOVA1 Antibody (orb19796)
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