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NRAS Antibody

SKU: orb19888

Description

Goat polyclonal antibody to NRAS

Research Area

Cancer, Epigenetics

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:64000, WB: 0.3-1μg/ml
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration, 2-3ug/ml.WB: Approx. 21kDa band observed in Human lysates of cell line A431 (calculated MW of 21.2kDa according to NP_002515.1). In transfected HEK293 transiently expressing NRAS bands of approx. 21kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetNRAS
Protein SequenceRMKKLNSSDDGTQ
Molecular Weight21.2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti NRAS antibody, anti neuroblastoma RAS viral (v-ras) oncogene homolog antibody, anti N-ras antibody, anti NRAS1 antibody, anti N-ras protein part 4 antibody, anti v-ras neuroblastoma RAS viral oncogene homolog antibody

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

NRAS Antibody (orb19888)

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100 μg
¥ 6,890.00
DispatchUsually dispatched within 5-10 working days
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