OCT4/POU5F1 (mouse) Antibody

SKU: orb125021

Description

Goat polyclonal antibody to POU5F1

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:16000, WB: 1-2 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Application Notes	WB: Approx 38-40kDa band observed in lysates of cell line NIH3T3 and approx. 45kDa in lysates of cell line HepG2 (calculated MW of 38.6kDa according to Human NP_002692.2 and 38.2kDa according to Mouse NP_038661.2). This molecular weight is routinely observed

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	OCT4 / POU5F1 (mouse)
Protein Sequence	DRPNAVKLEKVEP
Molecular Weight	38.2; 25.0
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti Oct3 antibody, anti Oct-3 antibody, anti Oct3/4 antibody, anti Oct-3/4 antibody, anti Oct4 antibody, anti Oct-4 antibody, anti Otf3 antibody, anti Otf-3 antibody, anti Otf3g antibody, anti Otf3-rs7 antibody, anti Otf4 antibody, anti Otf-4 antibody, anti POU domain, class 5, transcription factor 1 antibody, anti Pou5f1 antibody

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Western blot analysis of HepG2 cell lysate using POU5F1 antibody

Western blot analysis of NIH3T3 cell lysate using POU5F1 antibody

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Gene Symbol

OCT4 / POU5F1 (mouse)

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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