PIR51/RAD51AP1 Antibody

SKU: orb18781

Description

Goat polyclonal antibody to Rad51

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

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Tested Applications	ELISA, FC, IF, IHC
Dilution range	ELISA: 1:32000, WB: 0.2ug/ml, IHC-P: 4-6µg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 25kDa band in Human Testis lysates at 0.2 μg/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 39kDda according to NP_006470. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	PIR51 / RAD51AP1
Molecular Weight	38.5; 36.8
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Disclaimer	For research use only

Alternative Names

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anti DNA repair protein RAD51 homolog 1 antibody, anti DNA repair protein rhp51 antibody, anti Homolog of E coli RecA antibody, anti homolog of S cerevisiae RAD51 antibody, anti HRAD51 antibody, anti HsRad51 antibody, anti Rad 51 antibody, anti RAD51 antibody, anti RAD51 homolog antibody, anti RAD51 homolog S. cerevisiae antibody, anti RAD51 S cerevisiae homolog antibody, anti RAD51, S. cerevisiae, homolog of antibody, anti RAD51A antibody, anti RECA antibody, anti RecA homolog E. coli antibody, anti RecA like protein antibody, anti RecA, E. coli, homolog of antibody

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Immunohistochemical staining of Human Testis using Rad51 antibody

Quick Database Links

Gene Symbol

PIR51 / RAD51AP1

NCBI Gene ID

10635

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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