Pyruvate dehydrogenase/PDHX Antibody

SKU: orb18997

Description

Goat polyclonal antibody to PDHX

Research Area

Epigenetics

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:32000, WB: 0.1-1 μg/ml
Reactivity	Human
Predicted Reactivity	Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3ug/ml.WB: Approx 50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1 μg/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Pyruvate dehydrogenase / PDHX
Molecular Weight	54.2; 51.5; 29.9
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti PDHX antibody, anti pyruvate dehydrogenase complex, component X antibody, anti E3BP antibody, anti OPDX antibody, anti PDX1 antibody, anti proX antibody, anti DLDBP antibody, anti E3-binding protein antibody, anti pyruvate dehydrogenase complex, lipoyl-containing component X antibody, anti dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody, anti pyruvate dehydrogenase complex, E3-binding protein subunit antibody, anti pyruvate dehydrogenase protein X component, mitochondrial antibody

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orb18997 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Pyruvate dehydrogenase / PDHX

NCBI Gene ID

8050

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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