Goat Human IgE heavy (epsilon chain), HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum Antibody

SKU: orb700311

Description

Goat polyclonal antibody against Goat anti-Human IgE heavy (epsilon chain) conjugated to HRP

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution Range	The optimal working dilution should be determined by the investigator
Reactivity	Human
Application Notes	No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis. HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Purified human IgE
Purification	Immunogen affinity purified goat IgG.
Conjugation	HRP

Storage & Handling

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Storage	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/Appearance	Lyophilized
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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