HLA-DP + HLA-DQ + HLA-DR Antibody (MHC II)

SKU: orb386111

Description

This mAb reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some auto-immune diseases are associated with certain class II alleles.

Images & Validation

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Tested Applications	ELISA, FACS, IF, IHC-P, WB
Dilution range	ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/ million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	CR3/43
Immunogen	Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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HLA-DP + HLA-DQ + HLA-DR Antibody (MHC II)

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

Western blot testing of human Ramos cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

Western blot testing of human spleen cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1ug/10^6 cells (blocked with goat sera); Red = isotype control, Blue = HLA-DP/DQ/DR antibody.

SDS-PAGE analysis of purified, BSA-free HLA-DP/DQ/DR antibody (clone CR3/43) as confirmation of integrity and purity.

Quick Database Links

UniProt

P04440, P01920, P01908, P01909, P01903

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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