Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb347347

Description

Human IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Human
Application Notes	Anti-Human IgG (H&L) Alkaline Phosphatase has been tested by western blot and assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate ) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:9,000 of the reconstitution concentration is suggested for this product.

Key Properties

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Antibody Type	Secondary Antibody
Host	Gallus
Clonality	Polyclonal
Isotype	IgG
Immunogen	Human IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Chicken Serum, Human IgG and Human Serum.
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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chicken anti-Human IgG alkaline phosphatase conjugated Antibody, chicken anti-Human IgG Antibody alk phos conjugation

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Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Western Blot of Chicken anti-Human IgG Antibody Alkaline Phosphatase Conjugated Lane 1: Human IgG Load: 100 ng per lane Secondary antibody: Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT Block: orb348637 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 kDa

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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