ICOS Ligand Antibody / ICOS-L / B7RP-1

SKU: orb607112

Description

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). [UniProt]

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	FACS, IF, IHC-P
Dilution range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	Optimal dilution of the ICOS Ligand antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	ISLG-1
Immunogen	A recombinant fragment of human ICOSLG / B7RP-1 protein within amino acids 23-149 was used as the immunogen for the ICOS Ligand antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human colon carcinoma with ICOS Ligand antibody (clone ISLG-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Immunofluorescence staining of human U937 cells with ICOS Ligand antibody (green, clone ISLG-1) and Reddot nuclear stain (red).

Flow cytometry testing of human U937 cells with ICOS Ligand antibody (clone ISLG-1); Red=isotype control, Blue= ICOS Ligand antibody.

Quick Database Links

UniProt

O75144

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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