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IGF2 Antibody (Center R54)
SKU: orb1938352
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:25 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB18750 |
| Target | This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2. |
| Molecular Weight | 20140 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−Insulin-like growth factor II, IGF-II, Somatomedin-A, Insulin-like growth factor II, Insulin-like growth factor II Ala-25 Del, Preptin, IGF2
Similar Products
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IGF2 Antibody (Center R54) [orb2997048]
FC, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
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All lanes: Anti-IGF2 Antibody (Center R54) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-IGF2 Antibody (Center R54) at 1:2000 dilution + mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
IGF2 Antibody (Center R54) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry. Clinical relevance has not been evaluated.
IGF2 Antibody (Center R54) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).
Overlay histogram showing Hela cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Quick Database Links
Gene Symbol
This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2.
UniProt
RefSeq (Protein):NP_001121070.1, NP_001007140.2, NP_000603.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001121070.1, NP_001007140.2, NP_000603.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IGF2 Antibody (Center R54) (orb1938352)
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