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Il1b Antibody
SKU: orb1272469
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-beta (murine IL-1 beta). |
| Target | Il1b |
| Purification | Anti-mIL-1 beta specific antibody was purified by affinity chromatography employing immobilized mIL-1 beta matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Il-1b, IL-1betaInterleukin-1 betaIL-1 beta
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To detect mIL-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine IL-1-beta (orb1272468) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1-beta.
To detect mIL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mIL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected mouse brain (polymorph layer of the dentate gyrus) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Il1b Antibody (orb1272469)
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