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LIF Antibody
SKU: orb1239553
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-LIF antibody (orb1239553) was raised against a peptide corresponding to 16 amino acids near the center of human LIF. The immunogen is located within amino acids 50-100 of LIF. |
| Target | LIF |
| Molecular Weight | Predicted: 22kDObserved: 26kD (Post-modifications: N-linked Glycosylations) |
| Purification | LIF Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | LIF Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF
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Western Blot Validation in Mouse 3T3 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1: 1 µg/mL, 2: 2 µg/mL, 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Cell Lines and Tissue. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Lung. Loading: 15 µg of lysates per lane. Antibodies: LIF orb1239553, (1: 1 µg/mL, 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of LIF in Mouse 3T3 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with orb1239553 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
LIF Antibody (orb1239553)
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