M Sirt3 Antibody (C-term)

SKU: orb1930161

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P, WB
Dilution range	WB: 1:2000, WB: 1:64000, WB: 1:2000, WB: 1:2000, IHC-P: 1:50~100
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB25945
Target	This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.
Molecular Weight	36615 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Alternative Names

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NAD-dependent protein deacetylase sirtuin-3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, mSIR2L3, Sirt3, Sir2l3

Quality Guarantee

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Sirt3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Anti-Sirt3 Antibody (C-term) at 1:2000 dilution + mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Sirt3 Antibody (C-term) at 1: 64000 dilution + mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Sirt3 Antibody (C-term) at 1:2000 dilution + CHO whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Mouse Sirt3 Antibody (C-term) at 1:2000 dilution + mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.

UniProt

Q8R104

RefSeq (Protein):NP_001171275.1, NP_001120823.1, NP_071878.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001171275.1, NP_001120823.1, NP_071878.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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