MAPK14 Antibody (Center T180/Y182)

SKU: orb1937427

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-P, WB
Dilution range	IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Zebrafish

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB30167
Target	This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-192 amino acids from the Central region of human MAPK14.
Molecular Weight	41293 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Alternative Names

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Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, CSBP, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha, Stress-activated protein kinase 2a, SAPK2a, MAPK14, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A

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Fluorescent confocal image of A549 cell stained with MAPK14 Antibody (Center T180/Y182). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MAPK14 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MAPK14 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.

MAPK14 Antibody (Center T180/Y182) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MAPK14 Antibody (Center T180/Y182) for immunohistochemistry. Clinical relevance has not been evaluated.

MAPK14 Antibody (Center T180/Y182) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow).

Quick Database Links

Gene Symbol

This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-192 amino acids from the Central region of human MAPK14.

UniProt

Q16539

RefSeq (Protein):NP_620581.1, NP_620583.1, NP_001306.1, NP_620582.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_620581.1, NP_620583.1, NP_001306.1, NP_620582.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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