MCP-1, Human, mAb MNA1 Antibody

SKU: orb1109055

Description

Mouse monoclonal antibody to MCP-1

Images & Validation

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Tested Applications	FC, Functional Studies, IA, IHC-Fr, IP, PS, WB
Dilution range	For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies- dilutions have to be optimized in user’s experimental setting.
Reactivity	Human
Application Notes	FC: Antibody MNA.1 stains intracellular MCP-1.For intracellular staining HUVEC cells were permeabilized with buffer containing- 0.1% saponin. The cells were fixed in 4% paraformaldehyde before staining. As negative control a corresponding isotype control antibody was used- (Ref.5). W: A reduced sample treatment and 15% SDS-Page was used. The band sizes are ~14 and 11 kDa (Ref.1). IHC-P: Tissue sections were fixed in formalin and pretreated with hydrogen peroxide to quench endogenous peroxidases. Antigen retrieval was performed by pressure cooking for 3 minutes in PBS. As negative control the MNA.1 antibody was omitted (Ref.7). IHC-F:- Tissue sections were air dried and pretreated with 3% hydrogen peroxide to quench endogenous peroxidases. As negative control the MNA.1 antibody was omitted (Ref.2). FS: Antibody MNA.1 inhibits migration of monocytes by neutralizing MCP-1(Ref.1). Read more...

Key Properties

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Clonality	Monoclonal
Isotype	Mouse IgG1
Immunogen	Recombinant human MCP-1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Buffer/Preservatives	0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Disclaimer	For research use only

Alternative Names

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C-C motif chemokine 2, CCL2, small-inducible cytokine A2, MCAF, HC11

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunoprecipitation