MCP-1, Mouse, mAb ECE.2 Antibody

SKU: orb1109187

Description

Mouse monoclonal antibody to MCP-1

Images & Validation

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Tested Applications	IHC-Fr, WB
Dilution range	Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Reactivity	Mouse
Application Notes	F: Sections (6 um) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37°C or o/n 4 °C and then incubated with 2 µg/ml antibody for 2h at 37°C. W: Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 µg/ml) for 20 min at RT. Read more...

Key Properties

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Clonality	Monoclonal
Isotype	Rat IgG1
Immunogen	Synthetic peptide corresponding to residues 102-130 of mouse MCP-1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Buffer/Preservatives	0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Disclaimer	For research use only

Alternative Names

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C-C motif chemokine 2, Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2, Sigje Gene name: Ccl2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-Fr

Immunohistochemistry Frozen

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