Monoamine oxidase A+B Polyclonal Antibody

Blank control: Hela.Primary Antibody (green line): Rabbit Anti-Monoamine oxidase A+B antibody.Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE. Dilution: 1 µg /test.Protocol. The cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.