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Mouse hnRNP M3/4 Monoclonal Antibody
SKU: orb1520146
Description
Research Area
Immunology & Inflammation, Metabolism Research
Images & Validation
−Item 1 of 3
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:1000; IP - 20 µl/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 2A6-2H3 |
| Immunogen | hnRNP M1-4 proteins |
| Target | hnRNP M3/4 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 40 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CEA receptor; CEAR; heterogeneous nuclear ribonucleoprotein M; heterogenous nuclear ribonucleoprotein M4; hnRNA-binding protein M4; hnRNP M; HNRNPM4; HNRPM; HNRPM4; HTGR1; N-acetylglucosamine receptor 1; NAGR1
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Mouse hnRNP M3/4 Monoclonal Antibody [orb1520145]
IP, WB
Human, Mouse
Mouse
Monoclonal
Unconjugated
100 μg (BSA-free)
Quality Guarantee
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Detection of mouse hnRNP M3/4 by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, CT26, CH27, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520146) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human hnRNP M3/4 by western blot. Samples: Whole cell lysate (5 µg) from HEK293T, LNCaP, A-549, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520146) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human hnRNP M3/4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520146) used for IP at 20 µl/mg lysate.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_112480.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Mouse hnRNP M3/4 Monoclonal Antibody (orb1520146)
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