Mouse anti Human CD3 FITC - CD28 PE

SKU: orb198156

Description

Mouse polyclonal antibody to CD28 (FITC, PE)

Images & Validation

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Tested Applications	FACS
Reactivity	Human
Application Notes	PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Isotype	IgG1 (F)/IgG1 (PE)
Immunogen	CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.
Purification	Protein A/G Chromatography

Storage & Handling

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Storage	Product should be stored at 4-8°C. DO NOT FREEZE
Buffer/Preservatives	Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Concentration	Titered for flow cytometry
Hazard Information	This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Disclaimer	For research use only

Alternative Names

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CD3 FITC - CD28 PE

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Quick Database Links

UniProt

P07766, P10747

UniProt Details

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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