Mouse PDZK1-interacting Protein 1 (PDZK1IP1) ELISA Kit

SKU: orb867004

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Description

Sandwich Mouse PDZK1IP1 ELISA Kit

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDZK1IP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDZK1IP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDZK1IP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDZK1IP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	13.3 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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MAP17 ELISA Kit, 17 kDa membrane associated protein ELISA Kit, DD96 ELISA Kit, Epithelial protein up regulated in carcinoma membrane associated protein 17 ELISA Kit, Membrane associated protein 17 ELISA Kit, PDZK1-interacting protein 1 ELISA Kit, Pdzk1ip1 ELISA Kit, RP1-18D14.5 ELISA Kit, SPAP ELISA Kit, ELISA Kit

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Mouse PDZK1-interacting Protein 1 (PDZK1IP1) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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