p27Kip1 Antibody

SKU: orb2637646

Description

This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

Research Area

Cancer Biology

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-3ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
Reactivity	Human, Mouse, Rat
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p27Kip1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	SX53G8
Immunogen	Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone SX53G8, green) and Phalloidin (red).

IHC staining of FFPE human prostate carcinoma tissue with p287Kip1 antibody (clone SX53G8). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with p287Kip1 antibody (clone SX53G8). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Intracellular FACS testing of human HeLa cells with Alexa Fluor 488 conjugated p27Kip1 antibody (clone SX53G8, green) and isotype control (gray).

Flow cytometry staining of PFA-fixed human MCF7 cells with p27Kip1 antibody; Red = isotype control, Blue = p27Kip1 antibody.

SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone SX53G8) as confirmation of integrity and purity.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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