PAFAH1B1 Antibody

SKU: orb1247928

Description

PAFAH1B1 Antibody

Research Area

Neuroscience

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Reactivity	Human, Rat
Application Notes	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in Human Ovary and in Rat Ovary lysates (calculated MW of 46.6kDa according to Human NP_000421.1 and to Rat NP_113951.1). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:Paraffin embedded Human Brain (Cortex), Thyroid and Pancreas. Recommended concentration: 3.75µg/mL. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: TGSVDQTVKVWECR
Target	PAFAH1B1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Disclaimer	For research use only

Alternative Names

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PAFAH1B1, LIS1, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, MDCR, PAFAH, lissencephaly 1 protein, Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD), platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa), LIS2, MDS,

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orb1247928 (3.75 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

orb1247928 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247928 (0.1 ug/ml) staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247928 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Quick Database Links

Gene Symbol

PAFAH1B1

UniProt

P43034

RefSeq (Protein):NP_000421.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000421.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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