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PAX8 Recombinant Rabbit Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | ICC, IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:50-100, IHC-F=1:400-800, ICC/IF=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 5F9 |
| Immunogen | KLH conjugated synthetic peptide derived from human PAX8 |
| Target | PAX8 |
| Molecular Weight | 48 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Paraformaldehyde-fixed, paraffin embedded (human thyroid gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PAX8) Monoclonal Antibody, Unconjugated (orb1291601) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (MOUSE thyroid gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PAX8) Monoclonal Antibody, Unconjugated (orb1291601) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
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Protocol Information
PAX8 Recombinant Rabbit Monoclonal Antibody (orb1291601)
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