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Description
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:1000-1:3000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:300-1:400 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
| Target | Paxillin |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti-Paxillin antibody at 1/2000 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294377 overnight at 4°C.

Immunohistochemical of Paraffin sections. Sample: mouse cervix. Paxillin antibody (orb1294377): 1-300.

orb1294377 anti-Paxillin antibody.
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Paxillin Rabbit Polyclonal Antibody (orb1294377)
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